We capitalize on the biocompatibility of cellulose in medical applications through modifications with antibodies, enzymes and other bioactive molecules.
We have introduced conjugation of short peptides to nanocelluloses for low cost and disposable sensors as well as supports for detection or separation of bioactive molecules. We aim at developing sustainable bioactive materials that can be safely disposed or regenerated. Specific topics that have been developed in our group include:
- immunoglobulin G binding and detection
- heparin, Avidin-Biotin and other complexes
- affibodies and C-reactive proteins
- role of ligand spacer on passivation, binding, kinetics, and mass transfer
- rapid immunoassays and diagnostics
Schematic illustration of the preparation of channels on nanocellulose-based nanopaper and passivation towards nonspecific hIgG
1) Solin K., Beaumont M., Rosenfeldt S., Orelma H., Borghei M., Bacher M., Opietnik M., Rojas O.J., Self-assembly of soft cellulose nanospheres into colloidal gel layers with enhanced protein adsorption capability for next-generation immunoassays, Small, 2020, 16, 2004702.
2) Solin K., Orelma H., Borghei M., Vuoriluoto M., Koivunen R., Rojas O.J., Two-dimensional antifouling fluidic channels on nanopapers for biosensing, Biomacromolecules, 2019, 20, 1036-1044.
3) Guo J., Liu D., Filpponen I., Johansson L-S., Malho J-M., Quraishi S., Liebner F., Santos H.A., Rojas O.J., Photoluminescent hybrids of cellulose nanocrystals and carbon quantum dots as cytocompatible probes for in vitro bio-imaging, Biomacromolecules, 2017, 18, 2045-2055.
4) Shen F., Rojas O.J., Genzer J., Gurgel P.V., Carbonell R.G., Affinity interactions of human immunoglobulin G with short peptides: role of ligand spacer on binding, kinetics, and mass transfer, Analytical and Bioanalytical Chemistry, 2016, 408, 1829-1841.